High-Throughput Sequencing

Question 1 of 4

Animation 17.2 Quiz

In the sequencing method described in the tutorial, what chemical signal is converted to a light signal?

Pyrophosphate, PP_i

dATP

dGTP

Oil

Correct. See Key Concept 17.1 Genomes Can Be Sequenced Rapidly

Incorrect. See Key Concept 17.1 Genomes Can Be Sequenced Rapidly

Animation 17.2 Quiz

Which of the following statements summarizes the basic process used to determine a DNA sequence in the method described in the tutorial?

DNA fragments on beads are exposed to all four dNTPs in the presence of DNA polymerase to determine how long the growing chain becomes.

DNA fragments on beads are exposed to one dNTP at a time in the presence of DNA polymerase to determine which dNTP is taken up by the growing chain.

DNA fragments on beads are cut one nucleotide at a time to determine which nucleotide is released.

DNA fragments on beads are cut many nucleotides at a time to determine which group of nucleotides is released.

Correct. See Key Concept 17.1 Genomes Can Be Sequenced Rapidly

Incorrect. See Key Concept 17.1 Genomes Can Be Sequenced Rapidly

Animation 17.2 Quiz

What is an important difference about each bead in the high-throughput method of sequencing?

Each bead contains a different number of DNA molecules bound to it.

Each bead gives off a different amount of light during the sequencing process.

Each bead contains a different fragment of DNA to be sequenced.

Each bead undergoes a different amount of amplification via PCR.

Correct. See Key Concept 17.1 Genomes Can Be Sequenced Rapidly

Incorrect. See Key Concept 17.1 Genomes Can Be Sequenced Rapidly

Animation 17.2 Quiz

Refer to the table below.

The table shows results following four rounds of reactions for one bead during a high-throughput sequencing job. What is the sequence of the DNA fragment attached to the bead?

5ʹ-TAAGCCC-3ʹ

3ʹ-ATTCGGG-5ʹ

5ʹ-TAGC-3ʹ

3ʹ-ATCG-5ʹ

Correct. See Key Concept 17.1 Genomes Can Be Sequenced Rapidly

Incorrect. See Key Concept 17.1 Genomes Can Be Sequenced Rapidly

Activity results are being submitted...

Chapter 1. High-Throughput Sequencing